Adsorption Fibronectin and Adhesion of Osteoblast Into Implants

Modification of the dental implant surface, either chemically or topographically, can optimize bone healing, promote accelerated osteogenesis, increase bone-implant contact and bonding strength. In this work was analyzed the influence of implant surface chemical composition, roughness and adsorption of fibronectin in the adhesion of osteoblasts cells. Two titanium dental implants (acid etching surface and acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine the influence on interaction with osteoblastic cells. Characterization techniques used showed that both implant surface have similar microstructural characteristics. Using spectrophotometry, it was found similarity in the adsorption of fibronectin in both surfaces (80%). Association indexes of osteoblastic cells in samples previously treated with FN showed percentages of associated cells significantly higher than samples without FN. The radioactivity values associated with the same samples expressed as cpm (counts per minute), suggest that the FN incorporation is an important determinant at the in vitro cytocompatibility of surfaces. To optimize and to accelerate the osseointegration process coating of titanium surfaces with FN proved to be a treatment option for dental implants.