B. Li, X. Liu, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China; C. Ding, Chinese Academy of Sciences, Shanghai Institute of Ceramics, Shanghai, China
In this work, a gentamicine loaded collagen layer was grafted on the plasma sprayed titania coating to improve its cytocompatibility and antibiosis. The titania coating was pretreated by sulfuric acid to induce the formation of hydroxyl groups which can covalently graft collagen and render the collagen stabile. The cytocompatibility of the titania coating grafted with collagen was investigated by in vitro cell culture, and the resistance against staphylococcus aureus was measured by Zone of Inhibition test. Results show that the titania coating grafted with collagen enhanced the cellular adhesion and proliferation, indicating improved cytocompatibility. The gentamicine released from collagen could sustain for as long as 30 days, which is long enough to prevent against the postoperative infection.
Keywords: titania, collagen, gentamicine, plasma spray
In this work, a gentamicine loaded collagen layer was grafted on the plasma sprayed titania coating to improve its cytocompatibility and antibiosis. The titania coating was pretreated by sulfuric acid to induce the formation of hydroxyl groups which can covalently graft collagen and render the collagen stabile. The cytocompatibility of the titania coating grafted with collagen was investigated by in vitro cell culture, and the resistance against staphylococcus aureus was measured by Zone of Inhibition test. Results show that the titania coating grafted with collagen enhanced the cellular adhesion and proliferation, indicating improved cytocompatibility. The gentamicine released from collagen could sustain for as long as 30 days, which is long enough to prevent against the postoperative infection.
