R. S. Lima, S. Dimitrievska, M. N. Bureau, B. R. Marple, National Research Council of Canada, Boucherville, QC, Canada; A. Petit, Lady Davis Institute for Medical Research, Montreal, QC, Canada; J. Antoniou, Jewish General Hospital, Montreal, QC, Canada
HVOF-sprayed biomedical coatings produced from nano-TiO2 and nano-TiO2+10wt%HA powders have been engineered as possible future alternatives to APS HA coatings. This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials, human mesenchymal stem cells (hMSCs) and osteoblast differentiated-hMSCs (hMSCs-ob) were cultured from 1 to 21 days on the surface of HVOF-sprayed nano TiO2 and nano TiO2+10wt%HA coatings. APS HA coatings and Ti-6Al-4V substrates were employed as controls. The active profiles of the hMSCs and hMSCs-ob were evaluated via (i) biochemical analysis (alkaline phosphatase activity (ALP), osteocalcin (OC), type I C-terminal collagen propeptide (CICP)), (ii) cytoskeleton organization (confocal microscopy) and (iii) cell/substrate interaction (SEM). The biochemical analysis indicted that the bioactivity of the hMSCs and hMSCs-ob on the surface of HA and nano TiO2+10wt%HA coatings exhibited similar levels. However, the cytoskeleton organization and the cell/substrate interaction evaluation demonstrated a higher degree of cell proliferation and attachment on the surface of the HVOF-sprayed nano TiO2+10wt%HA coatings. These results are considered promising for the development of biomedical coatings.
Summary: HVOF-sprayed biomedical coatings produced from nano-TiO2 and nano-TiO2+10wt%HA powders have been engineered as possible future alternatives to APS HA coatings. This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials, human mesenchymal stem cells (hMSCs) and osteoblast differentiated-hMSCs (hMSCs-ob) were cultured from 1 to 21 days on the surface of HVOF-sprayed nano TiO2 and nano TiO2+10wt%HA coatings. APS HA coatings and Ti-6Al-4V substrates were employed as controls. The active profiles of the hMSCs and hMSCs-ob were evaluated via (i) biochemical analysis (alkaline phosphatase activity (ALP), osteocalcin (OC), type I C-terminal collagen propeptide (CICP)), (ii) cytoskeleton organization (confocal microscopy) and (iii) cell/substrate interaction (SEM). The biochemical analysis indicted that the bioactivity of the hMSCs and hMSCs-ob on the surface of HA and nano TiO2+10wt%HA coatings exhibited similar levels. However, the cytoskeleton organization and the cell/substrate interaction evaluation demonstrated a higher degree of cell proliferation and attachment on the surface of the HVOF-sprayed nano TiO2+10wt%HA coatings. These results are considered promising for the development of biomedical coatings.
