Diamond-like carbon (DLC) films have several advantages in biomedical applications such as high hardness, chemical inertness and low friction. Furthermore, SS 316L substrates coated with DLC films have been reported to have a good biocompatibility and high corrosion resistance. In the present investigation, the coatings were deposited in an industrial facility onto a SS 316L substrate by means of closed field unbalanced magnetron sputter ion plating. The chemical bonding structures of the DLC films were characterized by means of Raman spectroscopy and the electrochemical behavior were evaluated by means of electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization in a  0.89% NaCl solution of pH 7.4  at a temperature of 37ºC, simulating the body fluid environment. The porosity and protective efficiency of DLC coatings were obtained using potentiodynamic polarization tests. Moreover, the delamination area and volume fraction of water uptake of DLC coatings as a function of immersion time were calculated using electrochemical impedance spectroscopy. The DLC films showed high impedance, high polarization resistance and high breakdown potential, which were attributed to the high sp³ content and the uniformity of these films. The excellent chemical inertness of the DLC films makes them promising corrosion resistant coating materials for such applications.