ADSC Cells with Different Medium In Polycaprolactone/ Hydroxyapatite Whiskers Scaffolds

The use of Adipose stem cells (ADSC) is thoroughly study for the application in tissue engineering. Adipose stem cells have an immense human source and can be differential in different cells; these factors provide interest in different research groups. Poly (ɛ- caprolactone) (PCL) is a synthetic polymer that exhibits both biocompatible and biodegradable properties, and it is approved by the Food and Drug Admistration (FDA) to be used as a biomaterial. To improve the conductivity, biodegradability and bioactivity are produced scaffolds with different materials, such as the PCL composite with hydroxyapatite whiskers. The use of hydroxyapatite whiskers (HAw), allow you to increase your strength, beyond these bioactive and submit a character driver. Key issues for successful tissue engineering of bone are proper vascular supply and the potential of scaffolds to support and enhance the differentiation of stem cells along the osteogenic lineage. In this study the main goal was to observe the ADSC cells in the polycaprolactone/hydroxyapatite whiskers scaffolds into two kind of medium. Was used a medium with osteogenic factors and the other without it.  The scaffolds showed non toxicity for the human ADSC, indicated by the cell viability and cell proliferation during 3, 7, 14 and 21 days. The cell morphology into the two kinds of medium shows different aspects, analyzed by SEM images.