W. Haider, N. Munroe, V. Tek, Y. Tang, A. McGoron, C. Pulletikurthi, P. K. S. Gill, S. Pandya, Florida International University, Miami, FL
Most implantable medical devices are expected to function in the body over an extended period of time. Therefore, immersion tests under simulated conditions can be useful for assessing the amount of metal ions released in situ. In this investigation, dissovled ions from as recieved binary and ternary Nitinol alloys in cell culture media were periodically measured under static and dynamic conditions. Endothelial cells were grown in aliquots of culture media obtained and the effect of dissovled ions on cell proliferation and viability of endothelial cells (HUVEC) was studied by SRB assays. The concentration of metal ions in the media was measured by inductively coupled plasma mass spectrometry (ICPMS). The surface morphology of the alloys before and after immersion was studied by SEM.
Summary: In this investigation, dissovled ions from as recieved binary and ternary Nitinol alloys in cell culture media were periodically measured under static and dynamic conditions. Endothelial cells were grown in aliquots of culture media obtained and the effect of dissovled ions on cell proliferation and viability of endothelial cells (HUVEC) was studied by SRB assays. The concentration of metal ions in the media was measured by inductively coupled plasma mass spectrometry (ICPMS).
