S. Xu, H. Xia, C. Zhang, Changhai Hospital, The Second Military Medical University, Shanghai, China
yAbstractzObjective: To assess the proliferation of 3T3 cells on biodegradable shape-memory polymers. Methods The 3T3 cells were performed primary culture and generation culture, then the cells were inoculated on two carriers, one is biodegradable shape-memory polymers, the other is naked dish, and different cell culture systems were established. By protracting the cell growth curve, colorimetric assay of MTT and doping of 3H-TdR, the activity, proliferation and DNA synthesis of 3T3 cells on biodegradable shape-memory polymers were observed, and the biocompatibility of polymers were assessed. Results There was no statistical significant difference of biological characters between the polymers group and naked dish group, including the activity, proliferation and metabolism of 3T3 cells. Conclusion The biodegradable shape-memory polymers have good compatibility.
yKey Wordsz Biodegradable shape-memory polymers; 3T3-Swiss albino GNM 9; Biocompatibility
Summary: We performed an in vitro experiment to assess the biocompatibility of a new bio-degradable shape memory polymer invented by ourselves. By protracting the cell growth curve, colorimetric assay of MTT and doping of 3H-TdR, the activity, proliferation and DNA synthesis of 3T3 cells on biodegradable shape-memory polymers were observed, and the biocompatibility of polymers were assessed.
