The alloy could be used in for example a hip prosthesis or a dental implant. For this reason, it is necessary for it to promote a high cell adhesion and cell differentiation to enhance osteointegration and bone remodelling. This work aims to improve the bioactivity of the alloy by means of a surface modification treatment which is divided in three steps: plasma cleaning, surface silanization and immobilization of H-RGD6 elastin-like polymer. The functionalized surfaces have been characterized by X-ray photoelectron spectroscopy (XPS), zeta potential and contact angle. Cell culture and inmunofluorescence microscopy images have demonstrate that surface modification has improved adhesion of osteoblasts cell type.
With the polymer H-RGD6 elastin-like polymer, which contains RGD sequences, the cell adhesion has been improved. On the other hand, we have not observed significant differences in the proliferation tests between the functionalized samples and the untreated ones. Moreover, the calcium deposition (mineralization) after 14 days of culture has been increased in the functionalized samples.
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