3D Widefield and Confocal Microscopy – Principles and Industrial Applications

Traditional Microscopy looks at the world in two dimensions and often leaves parts of the field of view out of focus. By acquiring and digitally combining data over a range of observation heights, a single image which brings topographical features entirely into focus can be created. Three-dimensional maps of the region of interest can also be rendered and analyzed for metrological information. This presentation will explain the principles of 3 dimensional widefield and confocal microscopy and compare the strengths and weaknesses of the two methods. Selected case studies will be shared to illustrate the capabilities of 3D microscopy and its usefulness as a tool for quality improvement.